HRK Universal One Step Seamless  Cloning Kit

︱Product Description   ----------------------------------------------------------------------------------------------------------

HRK Universal One-Step Seamless Cloning Kit enables rapid, straightforward, and highly efficient directional cloning of DNA fragments between 50 bp and 10 kb, allowing PCR products to be inserted into any desired site of any vector. In practice, the vector is linearized, and 15–25 bp sequences homologous to the vector ends are added to the 5′ termini of the insert-specific PCR primers, ensuring that both ends of the amplified product match the linearized vector termini. Following incubation with the recombinase at 50 °C for 20 min, directional cloning is achieved with a positive cloning rate exceeding 95%.

The kit includes 2×HRK Seamless Cloning Master Mix, which comprises recombinase, reaction buffer, and proprietary enhancers to improve recombination efficiency. It also supports the simultaneous assembly of up to five DNA fragments in a single tandem cloning reaction.

︱Data Display   -------------------------------------------------------------------------------------------------------------------

● Single-fragment one-step recombination

The HRK Universal One-Step Seamless Cloning Kit (HRK-C2603) was used to recombine single fragments of 1.5 kb, 3.5 kb, and 9 kb with linearized vectors, and the resulting products were transformed into competent cells. Sixteen single colonies were selected for PCR identification. The results showed that the HRK product could effectively clone single‑fragment genes of different lengths.

● Efficient multi-fragment assembly

The HRK Universal One-Step Seamless Cloning Kit (HRK-C2603) and a competitor product (Company S) were evaluated for their ability to assemble two, three, or five fragments with linearized vectors. Following transformation, sixteen single colonies per reaction were screened by PCR. As shown in the left and right panels (colony counts and positive rates, respectively), HRK exhibited superior performance in both metrics.

︱Ordering Information   --------------------------------------------------------------------------------------------------------

* For Research Use Only. Not for use in diagnostic procedures.